Coding

Part:BBa_K4661015:Design

Designed by: Vlad Batagui   Group: iGEM23_KULeuven   (2023-10-08)


Engineered HFBI-sfGFP fusion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 400
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primer sequences used to generate this part

pET29_Gibs_fwd AGTGGAGGTTCTGGAGGTAGCATGGTTAGCAAAGGTGAAG

pET29_Gibs_rev TGAAGTGTATATCTCCTTCttaaag

HFBI_mut_Gibs_fwd aaGAAGGAGATATACACTTCAATGTCCAACGGTAATGGCAACG

HFBI_mut_Gibs_rev GCTACCTCCAGAACCTCCACTTGCCCCCACCGCTG


We put a lot of attention into designing parts that are compatible with various cloning methods and deriving strategies that are as modular as possible, allowing for the swapping of different coding sequences from our small library of hydrophobins into the pET29 plasmid map. Therefore, we ensured that future teams and people in the broader community could use our part and easily make it compatible with their systems.


Source

Gibson assembly from basic parts.

HFBI: GenBank 2FZ6_A. Trichoderma reesei

References